Mechanism in rat Coronary artery role of stiM1 and Orai1-dependent store Operated Calcium influx in Vasoconstriction

نویسندگان

  • Alejandro Domínguez-Rodríguez
  • Ignacio Díaz
  • Eva Calderón-Sánchez
  • Juan Antonio Rosado
  • Antonio Ordóñez
  • Tarik Smani
چکیده

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Urotensin-II signaling mechanism in rat coronary artery: role of STIM1 and Orai1-dependent store operated calcium influx in vasoconstriction.

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A STIM1-dependent ‘trafficking trap’ mechanism regulates Orai1 plasma membrane residence and Ca2+ influx levels

The key proteins mediating store-operated Ca entry (SOCE) are the endoplasmic reticulum (ER) Ca sensor STIM1 and the plasma membrane Ca-selective channel Orai1. Here, we quantitatively dissect Orai1 trafficking dynamics and show that Orai1 recycles rapidly at the plasma membrane (Kex≃0.1 min−1), with ∼40% of the total Orai1 pool localizing to the plasma membrane at steady state. A subset of int...

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تاریخ انتشار 2012